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Catalog No: OKEH03567
Size:96T
Price: $600.00
SKU
OKEH03567
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DKK2 ELISA Kit (Mouse) (OKEH03567) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Dkk2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dkk2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.8% (n = 20)
Mean Inter-assay CV%: <= 10.3% (n = 20)
ELISA Kit Component
ComponentAmount
Dkk2 Microplate96 Wells (12 x 8 Well strips)
Dkk2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Dkk2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.94 pg/mL
SpecificityNatural and recombinant Mouse Dickkopf-related protein 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDkk2
Gene Full Namedickkopf WNT signaling pathway inhibitor 2
Alias Symbolsdickkopf 2;dickkopf homolog 1;dickkopf homolog 2;dickkopf-related protein 2;dkk-2;mDkk-2.
NCBI Gene Id56811
Protein NameQ9QYZ8
Description of TargetDickkopf-related protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9QYZ8
Protein Accession #NP_064661.2
Nucleotide Accession #NM_020265.4
Protein Size (# AA)259
Molecular Weight28 kDa
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