DKK1 Polyclonal Antibody (OABB00416)

Catalog No: OABB00416 (Formerly GWB-BBP339)

Size:100UG

Lane 1: U87 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: A549 Cell Lysate

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  Background: DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
• Reference: 1. Fedi, P., Bafico, A., Soria, A. N., Burgess, W. H., Miki, T., Bottaro, D. P., Kraus, M. H., Aaronson, S.
  A.Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of
  mammalian Wnt signaling.J. Biol. Chem. 274: 19465-19472, 1999.
  2. Roessler, E., Du, Y., Glinka, A., Dutra, A., Niehrs, C., Muenke, M.The genomic structure,
  chromosome location, and analysis of the human DKK1 head inducer gene as a candidate for
  holoprosencephaly. Cytogenet. Cell Genet. 89: 220-224, 2000.
  3. Tian, E., Zhan, F., Walker, R., Rasmussen, E., Ma, Y., Barlogie, B., Shaughnessy, J. D., Jr.The role
  of Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma.New
  Eng. J. Med. 349: 2483-2494, 2003
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Dickkopf-related protein 1(DKK1) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: DKK1
• Gene Full Name: dickkopf WNT signaling pathway inhibitor 1
• Alias Symbols: dickkopf 1 homolog;dickkopf-1 like;dickkopf-like protein 1;dickkopf-related protein 1;DKK-1;SK.
• NCBI Gene Id: 22943
• Protein Name: Dickkopf-related protein 1
• Description of Target: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
• Uniprot ID: O94907
• Molecular Weight: 28672 MW