Catalog No: OKEH04073
Size:96T
Price: $425.00
SKU
OKEH04073
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04073
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Dkk1 ELISA Kit (Mouse) (OKEH04073) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dkk1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dkk1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dkk1 captured in the well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 8.8% (n = 20)
Mean Inter-assay CV%: <= 11.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Dkk1 Microplate96 Wells (12 x 8 Well Strips)
Dkk1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Dkk1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity31.36 pg/mL
SpecificityNatural and recombinant Mouse Dickkopf-related protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolDkk1
Gene Full Namedickkopf WNT signaling pathway inhibitor 1
Alias Symbolsdickkopf homolog 1;dickkopf-1;dickkopf-like protein 1;dickkopf-related protein 1;dkk-1;mdkk-1.
NCBI Gene Id13380
Protein NameO54908
Description of TargetDickkopf-related protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/O54908
Protein Accession #NP_034181.2
Nucleotide Accession #NM_010051.3
Protein Size (# AA)272
Molecular Weight29 kDa
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