DISC1 Antibody (OAPB00558)

Catalog No: OAPB00558

Size:100UG

Western blot analysis of DISC1 in SK-N-SH cell lysate with DISC1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunocytochemistry of DISC1 in HeLa cells with DISC1 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. DISC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: DISC1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: DISC1 antibody was raised against an 18 amino acid synthetic peptide from near the center of human DISC1. The immunogen is located within amino acids 350 - 400 of DISC1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-DISC1 antibody is Catalog # OEPB02689
• Application Info: DISC1 antibody can be used for detection of DISC1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: Immunocytochemistry of DISC1 in HeLa cells with DISC1 antibody at 5 ug/mL.
• Reference: Millar JK, Wilson-Annan JC, Anderson S, et al. Disruption of two novel genes by a translocation co-segregating with schizophrenia. Hum. Mol. Genet.2000; 9:1415-23.;Morris JA, Kandpal G, Ma L, et al. DISC1 (Disrupted-in-schizophrenia 1) is a centrosome-associated protein that interacts with MAP1A, MIPT3, ATF4/5 and NUDEL: regulation and loss of interaction with mutation. Hum. Mol. Genet.2003; 12:1591-608.;Taya S, Shinoda T, Tsuboi D, et al. DISC1 regulates the transport of the NUDEL/LIS1/14-3-3e complex through kinesin-1. J. Neurosci.2007; 27:15-26.;Duan X, Chang JH, Ge S, et al. Disrupted-in-schizophrenia 1 regulates integration of newly generated neurons in the adult brain. Cell2007; 1146-58.

Target Info

• Gene Symbol: DISC1
• Gene Full Name: DISC1 scaffold protein
• Alias Symbols: C1orf136;disrupted in schizophrenia 1 protein;SCZD9.
• NCBI Gene Id: 27185
• Protein Name: Disrupted in schizophrenia 1 protein|disrupted in schizophrenia 1 protein isoform L [Homo sapiens]
• Description of Target: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.
• Uniprot ID: Q9NRI5
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_061132
• Molecular Weight: Predicted: 72, 92, 94 kDa Observed: 94 kDa