DIO3 Antibody - C-terminal region (OAAB06126)

Catalog No: OAAB06126

Size:400UL

Western blot analysis of DIO3 Antibody (C-term) in HepG2 cell lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC,IHC-P,WB
• Additional Information: .
  Cellular Location: Cell membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein.
  Tissue Location: Expressed in placenta and several fetal tissues.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DIO3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-278 amino acids from the C-terminal region of human DIO3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.45 mg/ml
• Application Info: WB: 1:2000

Target Info

• Gene Symbol: DIO3
• Alias Symbols: 5DIII, D3, deiodinase, iodothyronine type III, DIOIII, iodothyronine deiodinase, placental type, selenoprotein DIO3, thyroxine 5-deiodinase, thyroxine deiodinase type III (selenoprotein), TXDI3, type 3 DI, type 3 iodothyronine selenodeiodinase, Type III iodothyronine deiodinase, type-III 5' deiodinase.
• NCBI Gene Id: 1735
• Protein Name: Thyroxine 5-deiodinase|thyroxine 5-deiodinase [Homo sapiens]
• Description of Target: Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
• Uniprot ID: P55073
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001353,NP_001353.4
• Molecular Weight: 33947