DICER1 ELISA Kit (Human) (OKCD09262)

Catalog No: OKCD09262

Size:96WELLS

DICER1 ELISA Kit (Human) (OKCD09262) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dicer 1, Ribonuclease Type III (DICER1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dicer 1, Ribonuclease Type III (DICER1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dicer 1, Ribonuclease Type III (DICER1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dicer 1, Ribonuclease Type III (DICER1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dicer 1, Ribonuclease Type III (DICER1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DICER1 Microplate: 96 Wells (12 x 8 Well strips)
  • DICER1 Lyophilized Standard: 2
  • 100X DICER1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.112ng/mL

Target Info

• Gene Symbol: DICER1
• Gene Full Name: dicer 1, ribonuclease III
• Alias Symbols: aviD;DCR1;Dicer;dicer 1, double-stranded RNA-specific endoribonuclease;dicer 1, ribonuclease type III;Dicer1, Dcr-1 homolog;Dicer1e;endoribonuclease Dicer;GLOW;helicase MOI;helicase with RNAse motif;HERNA;K12H4.8-LIKE;MNG1;RMSE2.
• NCBI Gene Id: 23405
• Protein Name: Endoribonuclease Dicer
• Description of Target: Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
• Uniprot ID: Q9UPY3
• Protein Accession #: NP_001182502.1
• Nucleotide Accession #: NM_001195573.1