Dicer Antibody (OASE00264)

Catalog No: OASE00264

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone S207-27. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody at 1:500, 1:1000, and 1:2000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone S207-27. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRP4 (Extracellular) Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S167-7
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, ICC, IF
• Additional Information: Background Info: Detects ~215kDa.
  Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate
  microRNAs (miRNAs) (1). After long primary transcript
  pri-miRNAs are processed to stem-looped pre-miRNAs by
  Drosha (2), pre-miRNAs are transported to the cytoplasm
  and further processed by Dicer to produce 22-nucleotide
  mature miRNAs (3). The mature miRNA then becomes a
  part of the RNA-Induced Silencing Complex (RISC) and
  can bind to the 3' UTR of the target mRNA (3)
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Dicer in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~215kDa.
• Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60.
  2. Lee Y., et al. (2003) Nature 425: 415-9.
  3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-
  108.
  
• Description: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)

Target Info

• Gene Symbol: Dicer
• Gene Full Name: dicer 1, ribonuclease type III
• Alias Symbols: Dicer1, DCR, DCR1, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI, KIAA0928, Endoribonuclease Dicer
• NCBI Gene Id: 192119
• Uniprot ID: Q8R418
• Protein Accession #: NP_683750.2