Dicer Antibody : Biotin (OASE00264-Biotin)

Catalog No: OASE00264-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S167-7
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, ICC
• Additional Information: Background Info: Detects ~215kDa.
  Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate
  microRNAs (miRNAs) (1). After long primary transcript
  pri-miRNAs are processed to stem-looped pre-miRNAs by
  Drosha (2), pre-miRNAs are transported to the cytoplasm
  and further processed by Dicer to produce 22-nucleotide
  mature miRNAs (3). The mature miRNA then becomes a
  part of the RNA-Induced Silencing Complex (RISC) and
  can bind to the 3' UTR of the target mRNA (3)
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Dicer in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer accession number Q8R418.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1ug/ml WB
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60.
  2. Lee Y., et al. (2003) Nature 425: 415-9.
  3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-
  108.
  

Target Info

• Gene Symbol: Dicer1
• Gene Full Name: dicer 1, ribonuclease type III
• Alias Symbols: Dice, D12Ertd7, D12Ertd7e, mKIAA0928, 1110006F08Rik
• NCBI Gene Id: 192119
• Uniprot ID: Q8R418
• Protein Accession #: NP_683750.2