Catalog No: OKDD00229 (Formerly GWB-KBBSV3)
Size:96 Wells
Price: $800.00
SKU
OKDD00229
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Datasheets/ManualsPrintable datasheet for DIAPH1 ELISA Kit (Human) (OKDD00229)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology DIAPH1 ELISA Kit (Human) (OKDD00229) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for DIAPH1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DIAPH1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DIAPH1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DIAPH1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DIAPH1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-DIAPH1 Microplate96 Wells (12 x 8 Well Strips)
DIAPH1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X DIAPH1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 0.051 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of DIAPH1. No significant cross-reactivity or interference between DIAPH1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolDIAPH1
Gene Full Namediaphanous related formin 1
Alias SymbolsDFNA1;DIA1;Diaphanous-related formin-1;DRF1;hDIA1;LFHL1;protein diaphanous homolog 1;SCBMS.
NCBI Gene Id1729
Protein NameProtein diaphanous homolog 1
Description of TargetActs in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed:20937854, PubMed:21834987). Plays a role in brain development (PubMed:24781755).
Uniprot IDO60610
Protein Accession #NP_001073280.1
Nucleotide Accession #NM_001079812.2
Protein Size (# AA)1272
Molecular Weight141 kDa
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