DHX9 Antibody - N-terminal region (OAAB17966)

Catalog No: OAAB17966

Size:400ul

DHX9 Antibody (N-term) (Cat. #OAAB17966) western blot analysis in K562 and 293 cell line lysates (35ug/lane).
This demonstrates the DHX9 antibody detected the DHX9 protein (arrow).
Dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded H. colon section using DHX9 Antibody (N-term)(Cat#OAAB17966). OAAB17966 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. breast section using DHX9 Antibody (N-term)(Cat#OAAB17966). OAAB17966 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of A549 cells stained with DHX9 Antibody (N-term)(Cat#OAAB17966). OAAB17966was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Product Info

• Predicted Species Reactivity: Cow
• Product Format: Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DHX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DHX9.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Cow
• Application Info: IF: 1:25
  IHC-P: 1:25
  WB: 1:1000
• Reference: Lee C.-G., et al. J. Biol. Chem. 268:16822-16830(1993).
  Abdelhaleem M.M., et al. J. Immunol. 156:2026-2035(1996).
  Zhang S., et al. J. Biol. Chem. 272:11487-11494(1997).
  Goshima N., et al. Nat. Methods 5:1011-1017(2008).
  Gregory S.G., et al. Nature 441:315-321(2006).

Target Info

• Gene Symbol: DHX9
• Alias Symbols: LKP, RHA, DDX9, NDH2, NDHII
• NCBI Gene Id: 1660
• Description of Target: Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
• Uniprot ID: Q08211, Q28141
• Protein Accession #: NP_001348.2
• Molecular Weight: 140958 Da