DHX30 Antibody - N-terminal region (ARP36425_P050)

Catalog No: ARP36425_P050

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WB Suggested Anti-DHX30 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate

Rabbit Anti-DHX30 Antibody
Catalog Number: ARP36425_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DHX30
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: AASRDLLKEFPQPKNLLNSVIGRALGISHAKDKLVYVHTNGPKKKKVTLH
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-DHX30 (ARP36425_P050) antibody is Catalog # AAP36425 (Previous Catalog # AAPP08481)
• Reference: Zhou,Y., (2008) Virology 372 (1), 97-106

Target Info

• Gene Symbol: DHX30
• Gene Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 30
• Alias Symbols: DDX30, RETCOR, NEDMIAL
• NCBI Gene Id: 22907
• Protein Name: Putative ATP-dependent RNA helicase DHX30
• Description of Target: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DHX30 is a member of this family. The protein has 97% sequence identity with the mouse HELG protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein has 97% sequence identity with the mouse HELG protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Uniprot ID: Q7L2E3
• Protein Accession #: NP_055781
• Nucleotide Accession #: NM_014966
• Protein Size (# AA): 1155
• Molecular Weight: 129kDa
• Protein Interactions: SUMO2; SUMO3; IVNS1ABP; STAU1; GRSF1; UBC; SUMO1; NEDD8; Fbxl16; WWOX; SUZ12; RNF2; EZH2; NEDD4; TARDBP; HNRNPD; EIF2AK2; SKIL; FN1; ESR1; NDUFA2; ILF3; GNAI2; RAB31; GRK5; CAND1; COPS5; CUL1; CUL3; CUL4B; CUL5; SIRT7; HNRNPA1; HDGF; DHX58; OAS3; Sgol2; Y