DHPS ELISA Kit (Human) (OKWB00169)

Catalog No: OKWB00169

Size:96 Wells

Human DHPS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-104%	86-105%	88-104%	87-101%
  EDTA Plasma (n=5)	85-100%	83-99%	82-98%	86-99%
  Heparin Plasma (n=5)	83-96%	82-97%	83-99%	84-93%

• ELISA Kit Principle: Aviva Systems Biology DHPS ELISA Kit (Human) (OKWB00169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DHPS has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DHPS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DHPS captured in the well.
• ELISA Kit Range: 0.313 - 20 ng/mL
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-99	92
  EDTA Plasma (n=5)	86-105	96
  Heparin Plasma (n=5)	85-104	95

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • DHPS Microplate: 96 Wells (12 x 8 Well strips)
  • DHPS Lyophilized Standard: 2 x 20 ng
  • 100X DHPS Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DHPS
• Alias Symbols: DS, DHS, MIG13, NEDSSWI
• NCBI Gene Id: 1725
• Protein Name: Deoxyhypusine synthase
• Description of Target: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
• Uniprot ID: P49366
• Protein Accession #: NP_001193903
• Nucleotide Accession #: NM_001206974