DHH ELISA Kit (Mouse) (OKEH03264)

Catalog No: OKEH03264

Size:96 Wells

Mouse DHH ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DHH ELISA Kit (Mouse) (OKEH03264) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dhh has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Dhh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dhh captured in well.
• ELISA Kit Range: 0.156 - 10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.3% (n = 20)
  Mean Inter-assay CV%: <= 8.2% (n = 20)
• ELISA Kit Component:
  • Anti-Dhh Microplate: 96 Wells (12 x 8 Well strips)
  • Dhh Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Dhh Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.088 ng/mL
• Specificity: Natural and recombinant Mouse Desert hedgehog protein
• Publications:

  VEGF-C mediates tumor growth and metastasis through promoting EMT-epithelial breast cancer cell crosstalk. Oncogene. (2020) 33299122

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DHH
• Gene Full Name: desert hedgehog
• Alias Symbols: C78960
• NCBI Gene Id: 13363
• Protein Name: Desert hedgehog protein(DHH)
• Description of Target: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.
• Uniprot ID: Q61488
• Protein Accession #: NP_031883.1
• Nucleotide Accession #: NM_007857.5
• Protein Size (# AA): 396
• Molecular Weight: 44 kDa