Catalog No: OKEH02539
Size:96T
Price: $600.00
SKU
OKEH02539
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology DHEA sulfate ELISA Kit (OKEH02539) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-DHEA sulfate antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DHEA sulfate and incubated. The DHEA sulfate found in the sample or standards competes with the biotinylated DHEA sulfate for limited binding sites on the immobilized anti-DHEA sulfate antibody. Excess unbound biotinylated DHEA sulfate and sample or standard DHEA sulfate is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DHEA sulfate captured in the well and inversely proportional to the amount of DHEA sulfate which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 99% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.2% (n = 20) Mean Inter-assay CV%: < 8.0% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Target Abbr: DHEAS Target Name: DHEA sulfate | ||||||||||||||||||||||
:: | Pubchem: 12594 | ||||||||||||||||||||||
:: | Chemical formula: C19H28O5S | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.054 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant General DHEA sulfate | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Alias Symbols | DHEAS, DHEA sulfate, DHEAS, Dehydroepiandrosterone sulfate |
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Protein Name | DHEA sulfate |
Description of Target | Dehydroepiandrosterone sulfate or DHEA sulfate is a metabolite of dehydroepiandrosterone (DHEA) produced by the addition of a sulfate group, catalyzed by the sulfotransferase enzymes SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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