Catalog No: OKEH02539
Size:96 Wells
Price: $600.00
SKU
OKEH02539
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DHEA sulfate ELISA Kit (OKEH02539) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-DHEA sulfate antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DHEA sulfate and incubated. The DHEA sulfate found in the sample or standards competes with the biotinylated DHEA sulfate for limited binding sites on the immobilized anti-DHEA sulfate antibody. Excess unbound biotinylated DHEA sulfate and sample or standard DHEA sulfate is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DHEA sulfate captured in the well and inversely proportional to the amount of DHEA sulfate which was contained in the sample or standard.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-DHEA sulfate Microplate96 Wells (12 x 8 Well strips)
DHEA sulfate Lyophilized Standard2 x 10 ng
Sample Diluent1 x 20 mL
100X DHEA sulfate-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: DHEAS
Target Name: DHEA sulfate
::Pubchem: 12594
::Chemical formula: C19H28O5S
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.054 ng/mL
SpecificityNatural and recombinant General DHEA sulfate
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsDHEAS, DHEA sulfate, DHEAS, Dehydroepiandrosterone sulfate
Protein NameDHEA sulfate
Description of TargetDehydroepiandrosterone sulfate or DHEA sulfate is a metabolite of dehydroepiandrosterone (DHEA) produced by the addition of a sulfate group, catalyzed by the sulfotransferase enzymes SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.
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