Catalog No: OKEH02530
Size:96 Wells
Price: $525.00
SKU
OKEH02530
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DHEA ELISA Kit (OKEH02530) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-DHEA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DHEA and incubated. The DHEA found in the sample or standards competes with the biotinylated DHEA for limited binding sites on the immobilized anti-DHEA antibody. Excess unbound biotinylated DHEA and sample or standard DHEA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DHEA captured in the well and inversely proportional to the amount of DHEA which was contained in the sample or standard.
ELISA Kit Range0.312 - 20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-DHEA Microplate96 Wells (12 x 8 Well strips)
DHEA Lyophilized Standard2 x 20 ng
Sample Diluent1 x 20 mL
100X DHEA-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: DHEA
Target Name: Dehydroepiandrosterone
::Pubchem: 5881
::Chemical formula: C19H28O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.091 ng/mL
SpecificityNatural and recombinant General Dehydroepiandrosterone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsDHEA, Dehydroepiandrosterone, DHEA
Protein NameDHEA
Description of Target5-Dehydroepiandrosterone (5-DHEA) is a 19-carbon endogenous steroid hormone. It is the major secretory steroidal product of the adrenal glands and is also produced by the gonads and the brain. DHEA is the most abundant circulating steroid in humans. DHEA has been implicated in a broad range of biological effects in humans and other mammals. It acts on the androgen receptor both directly and through its metabolites, which include androstenediol and androstenedione, which can undergo further conversion to produce the androgen testosterone and the estrogens, including estrone, estradiol, and estriol. DHEA is also a potent sigma-1 agonist. It is considered a neurosteroid.
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