DHCR24 Antibody (OAGA02194)

Catalog No: OAGA02194

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
Seladin 1 antibody
OAGA02194 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA02194 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Seladin 1 (OAGA02194) antibody at 1:100 dilution.

Seladin 1 antibody [N1C2] detects Seladin 1 protein at cytoplasm on rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
Seladin 1 antibody [N1C2] (OAGA02194) diluted at 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Seladin 1 (OAGA02194) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Seladin 1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.71mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: DHCR24
• Gene Full Name: 24-dehydrocholesterol reductase
• Alias Symbols: DCE, SELADIN1, Nbla03646, seladin-1
• NCBI Gene Id: 1718
• Description of Target: This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
• Uniprot ID: Q15392