DGKA ELISA Kit (Rat) (OKEH03740)

Catalog No: OKEH03740

Size:96T

Rat DGKA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DGKA ELISA Kit (Rat) (OKEH03740) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dgka has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Dgka is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dgka captured in well.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Component:
  • Dgka Microplate: 96 Wells (12 x 8 Well strips)
  • Dgka Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Dgka Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.317 ng/mL
• Specificity: Natural and recombinant Rat Diacylglycerol kinase alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Dgka
• Gene Full Name: diacylglycerol kinase, alpha
• Alias Symbols: 80 kDa diacylglycerol kinase;DAG kinase alpha;Dagk1;DGK-alpha;diacylglycerol kinase alpha;diglyceride kinase alpha.
• NCBI Gene Id: 140866
• Protein Name: P51556
• Description of Target: Diacylglycerol kinase alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P51556
• Protein Accession #: NP_542965.1
• Nucleotide Accession #: NM_080787.1
• Protein Size (# AA): 727
• Molecular Weight: 82 kDa