DGKA Antibody - C-terminal region (OAAB17490)

Catalog No: OAAB17490

Size:400UL

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2- acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.

Western blot analysis of anti-DGKA Antibody (C-term) (Cat.# OAAB17490) in HL60 cell line lysates (35ug/lane). DGKA (arrow) was detected using the purified Pab.

DGKA is supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Western blot
• Additional Information: Tissue Location: Expressed in lymphocytes.
  Cellular Location: Cytoplasm, cytosol
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DGKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598-628 amino acids from the C-terminal region of human DGKA.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Varies by lot. See vial for concentration.
• Sample Type Confirmation:

  DGKA is supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:1000

Target Info

• Gene Symbol: DGKA
• Gene Full Name: diacylglycerol kinase alpha
• Alias Symbols: 80 kDa diacylglycerol kinase;DAG kinase alpha;DAGK;DAGK1;DGK-alpha;diacylglycerol kinase alpha;diacylglycerol kinase, alpha 80kDa;diglyceride kinase alpha.
• NCBI Gene Id: 1606
• Protein Name: NP_001336.2, NP_958852.1, NP_958853.1, NP_963848.1, P23743
• Description of Target: Diacylglycerol kinase alpha, diacylglycerol kinase alpha isoform a [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/P23743
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001336.2,NP_958852.1,NP_958853.1,NP_963848.1
• Molecular Weight: 82630