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Datasheets/Manuals | Printable datasheet for DFFB Antibody (OAPB00060) (OAPB00060) |
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Predicted Species Reactivity | Bovine|Human|Mouse|Rat |
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Product Format | Liquid. DFF40 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | DFF40 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD. The immunogen is located within amino acids 130 - 180 of DFF40. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-DFFB (OAPB00060) antibody is Catalog # OEPB02187 |
Application Info | DFF40 antibody can be used for detection of DFF40 by Western blot at 1:500 to 1:1000 dilution. A 40 kDa band can be detected. (Optimal dilution should be determined by user) |
Reference | Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 ;Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 ;Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40 ;Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6 |
Gene Symbol | Dffb |
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Gene Full Name | DNA fragmentation factor, beta subunit |
Alias Symbols | 40kD;40kDa;5730477D02Rik;C;CA;CAD;caspase-activated deoxyribonuclease;caspase-activated DNase;CPAN;DFF40;DFF-40;Didf;Didff;DNA fragmentation factor 40 kDa subunit;DNA fragmentation factor subunit beta;DNA fragmentation factor, 40 kD, beta subunit;DNase inhibited by DNA fragmentation factor. |
NCBI Gene Id | 13368 |
Protein Name | DNA fragmentation factor subunit beta|DNA fragmentation factor subunit beta [Mus musculus] |
Description of Target | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
Uniprot ID | O54788 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_031885 |
Nucleotide Accession # | NM_004402.3 |
Molecular Weight | Predicted: 38 kDa Observed: 40 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "DFFB Antibody (OAPB00060)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Rat".
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How long will it take to receive "DFFB Antibody (OAPB00060)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "DFFB Antibody (OAPB00060)" provided in?
This item is provided in "Liquid. DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "DFFB Antibody (OAPB00060)"?
This target may also be called "40kD;40kDa;5730477D02Rik;C;CA;CAD;caspase-activated deoxyribonuclease;caspase-activated DNase;CPAN;DFF40;DFF-40;Didf;Didff;DNA fragmentation factor 40 kDa subunit;DNA fragmentation factor subunit beta;DNA fragmentation factor, 40 kD, beta subunit;DNase inhibited by DNA fragmentation factor." in publications.
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What is the shipping cost for "DFFB Antibody (OAPB00060)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "DFFB Antibody (OAPB00060)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "DFFB Antibody (OAPB00060)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 38 kDa Observed: 40 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "DFFB Antibody (OAPB00060)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Dffb"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Dffb"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Dffb"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Dffb"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Dffb"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Dffb"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.