DFF45 Antibody (OAPB00023)

Catalog No: OAPB00023 (Formerly GWB-B44FE9)

Size:100UG

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Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.

Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 μg/mL.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: DFF45 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-DFF45 antibody is Catalog # OEPB02152
• Application Info: DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blotat 1:1000 to 1:2000 dilution and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. (Optimal dilution should be determined by user)
• Reference: Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 ;Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 ;Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 ;Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6

Target Info

• Gene Symbol: DFFA
• Gene Full Name: DNA fragmentation factor subunit alpha
• Alias Symbols: DFF1;DFF45;DFF-45;DNA fragmentation factor 45 kDa subunit;DNA fragmentation factor subunit alpha;DNA fragmentation factor, 45kDa, alpha polypeptide;ICAD;inhibitor of CAD.
• NCBI Gene Id: 1676
• Protein Name: DNA fragmentation factor subunit alpha|DNA fragmentation factor subunit alpha isoform 1 [Homo sapiens]
• Description of Target: Inhibitor of the caspase-activated DNase (DFF40).
• Uniprot ID: O00273
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004392
• Molecular Weight: 45 kDa