Catalog No: OKEH05391
Size:96 Wells
Price: $525.00
SKU
OKEH05391
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Dexi ELISA Kit (Mouse) (OKEH05391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dexi has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dexi is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dexi captured in the well.
ELISA Kit Range0.625 - 40ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 93.5%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.3%, n = 20
Mean Inter-Assay Precision - <8.2%, n = 20
ELISA Kit Component
ComponentAmount
DEXI Microplate96 Wells (12 x 8 Well strips)
DEXI Lyophilized Standard2
100X Biotinylated DEXI Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.316 ng/mL
SpecificityNatural and recombinant Mouse Dexamethasone-induced protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDEXI
Gene Full NameDexamethasone-induced transcript
Alias SymbolsMyle, AI836170, AW413143, D16Bwg0586e, 1810029J14Rik
NCBI Gene Id58239
Protein NameDexamethasone-induced protein
Swissprot IdQ9WUQ7
Protein Accession #NP_067403.2
Nucleotide Accession #NM_021428.4
Protein Size (# AA)95
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express DEXI.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express DEXI.
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