Catalog No: OKCD09458
Size:96 Wells
Price: $750.00
SKU
OKCD09458
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology DEXI ELISA Kit (Human) (OKCD09458) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEXI has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEXI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEXI captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level DEXI. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level DEXI using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
DEXI Microplate96 Wells (12 x 8 Well strips)
DEXI Lyophilized Standard2
100X DEXI HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055ng/mL
Gene SymbolDEXI
Gene Full NameDexi homolog
Alias SymbolsMYLE
NCBI Gene Id28955
Protein NameDexamethasone-induced protein
Description of TargetPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Swissprot IdO95424
Protein Accession #NP_054734.2
Nucleotide Accession #NM_014015.3
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express DEXI.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express DEXI.
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