Catalog No: OKCD02383
Size:96WELLS
Price: $650.00
SKU
OKCD02383
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Datasheets/ManualsPrintable datasheet for Des ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-105%94-103%79-99%78-97%
EDTA Plasma (n=5)85-97%80-99%80-94%89-103%
Heparin Plasma (n=5)88-96%96-104%78-89%79-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmin (Des). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmin (Des). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmin (Des), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Desmin (Des) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range2.34-150ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)89-9995
EDTA Plasma (n=5)99-105102
Heparin Plasma(n=5)78-9484
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmin (Des) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmin (Des) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DES Microplate96 Wells (12 x 8 Well strips)
DES Lyophilized Standard2
100X Biotinylated DES Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.9 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Desmin (Des). No significant cross-reactivity or interference between Desmin (Des) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDES
Gene Full Namedesmin
Alias SymbolsDesmin
NCBI Gene Id64362
Protein NameDesmin
Description of TargetDesmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction.
Uniprot IDP48675
Protein Accession #NP_071976.1
Nucleotide Accession #NM_022531.1
Protein Size (# AA)469
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