Dengue virus NS3 Protein Antibody (OAGA07140)

Catalog No: OAGA07140

Size:100 ul

Sample (20 ug of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 ug BHK-21 whole cell extract
B. 30 ug whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 ug whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 ug whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 ug whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody diluted at 1:2000.
Blue: Hoechst 33342 staining.

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2) antibody at 1:2000 dilution.

Product Info

• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: FACS, ICC, IF, IHC, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.73 mg/ml
• Preservative: 0.025% ProClin 300
• Application Info: Suggested starting dilutions are as follows: FACS, ICC/IF: 1:100-1:2000, IHC, IHC-P, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Target Info

• Gene Symbol: Dengue virus NS3 protein
• Alias Symbols: polyprotein gene
• NCBI Gene Id: 1494449