SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OKCA00772
Size:96 Wells
Price: $754.00
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology DEK ELISA Kit (Human) (OKCA00772) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEK has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DEK captured in the well.
ELISA Kit Range23.44 pg/mL-1,500 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9590-100
EDTA Plasma (n=4)8884-92
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level DEK were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level DEK were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
DEK Microplate96 Wells (12 x 8 Well strips)
DEK Lyophilized Standard2
100X Biotinylated DEK Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity5.86 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolDEK
Gene Full NameDEK proto-oncogene
Alias SymbolsD6S231E
NCBI Gene Id7913
Protein NameProtein DEK
Description of TargetInvolved in chromatin organization.
Uniprot IDP35659
Protein Accession #NP_001128181.1
Nucleotide Accession #NM_001134709.1
Write Your Own Review
You're reviewing:DEK ELISA Kit (Human) (OKCA00772)
Your Rating
We found other products you might like!