DEK Antibody - N-terminal region (P100637_T100)

Catalog#: P100637_T100

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Guinea Pig, Horse, Human, Rabbit, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-136222 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DEK
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 78%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Rabbit: 85%; Rat: 85%
• Peptide Sequence: Synthetic peptide located within the following region: AAEGEGTPTQPASEKEPEMPGPREESEEEEDEDDEEEEEEEKEKSLIVEG
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-DEK (P100637_T100) antibody is Catalog # AAP30905 (Previous Catalog # AAPP01629)
• Target Reference: Orlic,M., et al., (2006) Genes Chromosomes Cancer 45 (1), 72-82
• Publications:

  Wang, N. et al. Down-regulation of HtrA1 activates the epithelial-mesenchymal transition and ATM DNA damage response pathways. PLoS One 7, e39446 (2012). WB, Cow, Guinea Pig, Horse, Human, Rabbit, Rat 22761798

• Gene Symbol: DEK
• Official Gene Full Name: DEK oncogene
• Alias Symbols: D6S231E
• NCBI Gene Id: 7913
• Protein Name: Protein DEK
• Description of Target: DEK was first identified in a fusion with the CAN nucleoporin protein in a specific subtype of acute myelogenous leukemia. DEK has also been shown to be an autoantigen in patients with pauciarticular onset juvenile rheumatoid arthritis. Further, the last 65 amino acids of DEK can partially reverse the mutation-prone phenotype of cells from patients with ataxia-telangiectasia
• Swissprot Id: P35659
• Protein Accession #: NP_003463
• Nucleotide Accession #: NM_003472
• Protein Size (# AA): 375
• Molecular Weight: 43kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express DEK.
• Protein Interactions: CUL3; SPOP; UBC; EED; IRF2BP2; GANAB; PTPN12; CSNK2A2; CSNK2A1; TP53BP1; ESR1; VTN; S100A9; RPL37A; DHX15; FTSJ3; WIBG; TMED9; EXOSC2; UTP14A; THRAP3; CAND1; COPS5; CDK2; YWHAZ; Cebpb; ELAVL1; SUMO1; SUMO2; RAD21; HDGF; KAT2B; EP300; CREBBP; HIST1H4A; HDA

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: DEK antibody tested with Human Jurkat Cells (P100637_T100)