Catalog No: OKEH08228
Size:96T
Price: $725.00
SKU
OKEH08228
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology DEGS1 ELISA Kit (Human) (OKEH08228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEGS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEGS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEGS1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=2.8% (n = 20)
Mean Inter-assay CV%: <=11.1% (n = 20)
ELISA Kit Component
ComponentAmount
DEGS1 Microplate96 Wells (12 x 8 Well strips)
DEGS1 Lyophilized Standard2
100X Biotinylated DEGS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).
::Subcellular Location: Mitochondrion Endoplasmic reticulum membrane Multi-pass membrane protein Membrane Lipid-anchor
::Unigene: Hs.299878
String: 9606.ENSP00000316476
::KEGG: hsa:8560
MIM: 615843
Pfam: PF00487
Reconstitution and StorageStore as indicated in product manual.
Sensitivity40.2pg/mL
SpecificityNatural and recombinant Human Sphingolipid delta(4)-desaturase DES1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDEGS1
Gene Full Namedelta 4-desaturase, sphingolipid 1
Alias Symbolscell migration-inducing gene 15 protein;Degenerative spermatocyte homolog 1;degenerative spermatocyte homolog 1, lipid desaturase;degenerative spermatocyte homolog, lipid desaturase;DEGS;DEGS-1;Des-1;DES1;dihydroceramide desaturase 1;FADS7;HLD18;membrane fatty acid (lipid) desaturase;membrane lipid desaturase;MIG15;migration-inducing gene 15 protein;MLD;sphingolipid delta 4 desaturase;sphingolipid delta(4)-desaturase 1;sphingolipid delta(4)-desaturase DES1.
NCBI Gene Id8560
Protein NameO15121
Description of TargetSphingolipid delta(4)-desaturase DES1
Uniprot IDhttps://www.uniprot.org/uniprot/O15121
Protein Accession #NP_003667.1
Nucleotide Accession #NM_003676.3
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