Catalog No: OKCD02992
Size:96WELLS
Price: $800.00
SKU
OKCD02992
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Datasheets/ManualsPrintable datasheet for DEFB4A ELISA Kit (Human) (OKCD02992)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 2 (DEFb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 2 (DEFb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 2 (DEFb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Beta 2 (DEFb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 2 (DEFb2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 2 (DEFb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
DEFB4A Microplate96 Wells (12 x 8 Well strips)
DEFB4A Lyophilized Standard2
100X DEFB4A HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 13.2 pg/mL
Gene SymbolDEFB4A|DEFB4B
Gene Full Namedefensin beta 4A|defensin beta 4B
Alias SymbolsBD-2;beta defensin 2;beta defensin-2;Beta-defensin 2;beta-defensin 4A;beta-defensin 4B;DEFB102;DEFB2;DEFB-2;DEFB4;DEFB4P;defensin, beta 2;defensin, beta 4;defensin, beta 4, pseudogene;HBD-2;SAP1;skin-antimicrobial peptide 1.
NCBI Gene Id100289462|1673
Protein NameBeta-defensin 4A
Description of TargetExhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells (PubMed:20068036).
Uniprot IDO15263
Protein Accession #NP_001192195.1
Nucleotide Accession #NM_001205266.1
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