DEFB4A ELISA Kit (Human) : 96 Wells (OKEH00376)

Catalog No: OKEH00376

Size:96T

Human DEFB4A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DEFB4A ELISA Kit (Human) (OKEH00376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB4A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for DEFB4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB4A captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.4% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • DEFB4A Microplate: 96 Wells (12 x 8 Well strips)
  • DEFB4A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated DEFB4A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.045 ng/mL
• Specificity: Natural and recombinant Human Beta-defensin 4A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DEFB4A, DEFB4B
• Gene Full Name: defensin beta 4A, defensin beta 4B
• Alias Symbols: BD-2;beta defensin 2;beta defensin-2;Beta-defensin 2;beta-defensin 4A;beta-defensin 4B;DEFB102;DEFB2;DEFB-2;DEFB4;DEFB4P;defensin, beta 2;defensin, beta 4;defensin, beta 4, pseudogene;HBD-2;SAP1;skin-antimicrobial peptide 1.
• NCBI Gene Id: 100289462, 1673
• Protein Name: O15263
• Description of Target: Beta-defensin 4A
• Uniprot ID: https://www.uniprot.org/uniprot/O15263
• Protein Accession #: NP_001192195.1
• Nucleotide Accession #: NM_001205266.1
• Protein Size (# AA): 64
• Molecular Weight: 7 kDa