DEFB3 ELISA Kit (Mouse) : 96 Wells (OKEH07209)

Catalog No: OKEH07209

Size:96T

Mouse DEFB3 ELISA Kit standard curve.

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology DEFB3 ELISA Kit (Mouse) (OKEH07209) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for DEFB3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 9.8% (n = 20)
• ELISA Kit Component:
  • DEFB3 Microplate: 96 Wells (12 x 8 Well strips)
  • DEFB3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated DEFB3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: This assay recognizes recombinant and natural mouse BD-3. No significant cross-reactivity or interference was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Defb3
• Gene Full Name: defensin beta 3
• Alias Symbols: BD-3;beta-defensin 3;Defensin, beta 3.
• NCBI Gene Id: 27358
• Protein Name: Q9WTL0
• Description of Target: Beta-defensin 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q9WTL0
• Protein Accession #: NP_038784.1
• Nucleotide Accession #: NM_013756.2
• Protein Size (# AA): 63
• Molecular Weight: 8 kDa