Catalog No: OKCD02106 (Formerly GWB-KBBMF1)
Size:96WELLS
Price: $750.00
SKU
OKCD02106
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Datasheets/ManualsPrintable datasheet for DEFB131 ELISA Kit (Human) (OKCD02106)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-97%89-98%93-105%96-105%
EDTA Plasma (n=5)79-94%93-101%92-103%79-103%
Heparin Plasma (n=5)91-101%80-96%93-102%84-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 131 (DEFb131). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 131 (DEFb131). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 131 (DEFb131), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Beta 131 (DEFb131) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)96-105101
EDTA Plasma (n=5)81-9288
Heparin Plasma (n=5)78-9891
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 131 (DEFb131) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 131 (DEFb131) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DEFB131 Microplate96 Wells (12 x 8 Well Strips)
DEFB131 Lyophilized Standard2 x 1 ng
100X Biotinylated DEFB131 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 5.6 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Defensin Beta 131 (DEFb131). No significant cross-reactivity or interference between Defensin Beta 131 (DEFb131) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDEFB131A
Gene Full Namedefensin beta 131A
Alias Symbolsbeta-defensin 131;beta-defensin 131A;beta-defensin 31;DEFB131;DEFB-31;defensin beta 131;Defensin, beta 131;defensin, beta 31.
NCBI Gene Id644414
Protein NameBeta-defensin 131A
Description of TargetHas antibacterial activity (Probable). Upon stimulation with lipoteichoic acid, promotes cytokines and chemokines production and secretion (PubMed:26649771).
Uniprot IDP59861
Protein Accession #NP_001035538.2
Nucleotide Accession #NM_001040448.2
Protein Size (# AA)70
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