DEFA6 ELISA Kit (Mouse) (OKCD01807)

Catalog No: OKCD01807 (Formerly GWB-KBBHN7)

Size:96WELLS

Mouse Defa6 more ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	84-103%	81-90%	84-94%	89-103%
  EDTA Plasma (n=5)	87-97%	80-92%	97-105%	78-89%
  Heparin Plasma (n=5)	87-94%	85-95%	89-96%	87-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 6, Paneth Cell Specific (DEFa6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 6, Paneth Cell Specific (DEFa6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 6, Paneth Cell Specific (DEFa6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Alpha 6, Paneth Cell Specific (DEFa6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	86-98	93
  Heparin Plasma (n=5)	80-99	79

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Alpha 6, Paneth Cell Specific (DEFa6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Alpha 6, Paneth Cell Specific (DEFa6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-DEFA6 Microplate: 96 Wells (12 x 8 Well Strips)
  • DEFA6 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated DEFA6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Defensin Alpha 6, Paneth Cell Specific (DEFa6). No significant cross-reactivity or interference between Defensin Alpha 6, Paneth Cell Specific (DEFa6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Defa6
• Gene Full Name: defensin, alpha, 6
• Alias Symbols: alpha-defensin 6/12;alpha-defensin, 6;cryptdin-2;Defcr;Defcr6;defensin related cryptdin 6;defensin-related cryptdin-6/12.
• NCBI Gene Id: 13240
• Protein Name: Alpha-defensin 6/12
• Description of Target: Has broad-spectrum antimicrobial properties. Has antibacterial activity against the Gram-positive bacterium L.monocytogenes EGD and the Gram-negative bacteria E.coli ML-35p and avirulent S.typhimurium 7953, but not against the mouse-virulent S.typhimurium 14028S. Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
• Uniprot ID: P50704
• Protein Accession #: NP_031878.1
• Nucleotide Accession #: NM_007852.1
• Protein Size (# AA): 93