DEFA1 ELISA Kit (Mouse) (OKCD07613)

Catalog No: OKCD07613

Size:96WELLS

DEFA1 ELISA Kit (Mouse) (OKCD07613) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 1, Neutrophil (DEFa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 1, Neutrophil (DEFa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 1, Neutrophil (DEFa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Defensin Alpha 1, Neutrophil (DEFa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Alpha 1, Neutrophil (DEFa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Alpha 1, Neutrophil (DEFa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • DEFA1 Microplate: 96 Wells (12 x 8 Well strips)
  • DEFA1 Lyophilized Standard: 2
  • 100X DEFA1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 13.3pg/mL

Target Info

• Gene Symbol: Defa1
• Gene Full Name: defensin, alpha 1
• Alias Symbols: alpha-defensin 1;cryptd;cryptdin-1;Defcr;Defcr1;defensin related cryptdin peptide 1;defensin-1;defensin-related cryptdin peptide.
• NCBI Gene Id: 13216
• Protein Name: Alpha-defensin 1
• Description of Target: Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.
• Uniprot ID: P11477
• Protein Accession #: NP_034161.2
• Nucleotide Accession #: NM_010031.2