DEDD Antibody (OAGA00930)

Catalog No: OAGA00930

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
OAGA00930 diluted at 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DEDD(OAGA00930) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2500.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  Positive Controls: 293T, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm, Nucleus, nucleolus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 129 and 223 of DEDD (Uniprot ID#O75618)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.42 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: DEDD
• Gene Full Name: Death effector domain-containing protein
• Alias Symbols: DEFT, KE05, DEDD1, FLDED1, CASP8IP1
• NCBI Gene Id: 9191
• Protein Name: Death effector domain-containing protein
• Uniprot ID: O75618
• Protein Accession #: NP_001034800.1
• Molecular Weight: 37 kDa