DDX58 Recombinant Protein (OPCD06694)

Catalog No: OPCD06694

DDX58 Recombinant Protein (OPCD06694) using SDS-PAGE

DDX58 Recombinant Protein (OPCD06694) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Asp550-Phe776
  Subcellular Location: Membrane, Cytoplasm
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 97%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: DDX58
• Gene Full Name: DExD/H-box helicase 58
• Alias Symbols: antiviral innate immune response receptor RIG-I;DEAD (Asp-Glu-Ala-Asp) box polypeptide 58;DEAD box protein 58;DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide;probable ATP-dependent RNA helicase DDX58;retinoic acid-inducible gene 1 protein;retinoic acid-inducible gene I protein;RIG1;RIGI;RIG-I;RIG-I-like receptor 1;RLR-1;RNA helicase RIG-I;SGMRT2.
• NCBI Gene Id: 23586
• Protein Name: Probable ATP-dependent RNA helicase DDX58
• Description of Target: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.
• Uniprot ID: O95786
• Protein Accession #: NP_055129.2
• Nucleotide Accession #: NM_014314.3
• Protein Size (# AA): Asp550~Phe776
• Molecular Weight: 30kDa