DDX5 Antibody (OACA07279)

Catalog No: OACA07279

Immunohistochemistry of paraffin-embedded human breast cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Immunohistochemistry of paraffin-embedded human tonsil at dilution 1:100

All lanes: Probable ATP-dependent RNA helicase DDX5 antibody at 3ug/ml
Lane 1: NIH3T3 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 70,61 kDa
Observed band size: 69 kDa

All lanes: Probable ATP-dependent RNA helicase DDX5 antibody at 3ug/ml
Lane 1: NIH3T3 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 70,61 kDa
Observed band size: 69 kDa

Immunohistochemistry of paraffin-embedded human breast cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Immunohistochemistry of paraffin-embedded human tonsil at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Probable ATP-dependent RNA helicase DDX5 protein (1-290AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"Comparative structural analysis of human DEAD-box RNA helicases." Schutz P., Karlberg T., van den Berg S., Collins R., Lehtio L., Hogbom M., Holmberg-Schiavone L., Tempel W., Park H.W., Hammarstrom M., Moche M., Thorsell A.G., Schuler H.PLoS ONE 5:E12791-E12791(2010). [2]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011). [3]"RNA helicase p68 (DDX5) regulates tau exon 10 splicing by modulating a stem-loop structure at the 5' splice site."Kar A., Fushimi K., Zhou X., Ray P., Shi C., Chen X., Liu Z., Chen S., Wu J.Y.Mol. Cell. Biol. 31:1812-1821(2011).

Target Info

• Gene Symbol: DDX5
• Gene Full Name: DEAD-box helicase 5
• Alias Symbols: ATP-dependent RNA helicase DDX5;DEAD (Asp-Glu-Ala-Asp) box helicase 5;DEAD (Asp-Glu-Ala-Asp) box polypeptide 5;DEAD box protein 5;DEAD box-5;DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD);G17P1;HLR1;HUMP68;p68;probable ATP-dependent RNA helicase DDX5;RNA helicase p68.
• NCBI Gene Id: 1655
• Protein Name: Probable ATP-dependent RNA helicase DDX5
• Description of Target: Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
• Uniprot ID: P17844
• Protein Accession #: NP_001307524
• Nucleotide Accession #: NM_001320595