DDX42 ELISA Kit (Chicken) (OKEH08050)

Catalog No: OKEH08050

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology DDX42 ELISA Kit (Chicken) (OKEH08050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDX42 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDX42 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DDX42 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.5% (n = 20)
  Mean Inter-assay CV%: <=6.4% (n = 20)
• ELISA Kit Component:
  • DDX42 Microplate: 96 Wells (12 x 8 Well strips)
  • DDX42 Lyophilized Standard: 2
  • 100X Biotinylated DDX42 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: ATP-dependent RNA helicase. Binds to RNA. May be involved in the survival of cells.
  Subcellular Location: Cytoplasm Nucleus
  Unigene: Gga.5156
  String: 9031.ENSGALP00000000810
  KEGG: gga:419959
  Pfam: PF00270
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken ATP-dependent RNA helicase DDX42
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DDX42
• Gene Full Name: DEAD-box helicase 42
• Alias Symbols: ATP-dependent RNA helicase DDX42;DEAD (Asp-Glu-Ala-Asp) box helicase 42;DEAD (Asp-Glu-Ala-Asp) box polypeptide 42;DEAD box protein 42.
• NCBI Gene Id: 419959
• Protein Name: Q5F485
• Description of Target: ATP-dependent RNA helicase DDX42
• Uniprot ID: https://www.uniprot.org/uniprot/Q5F485
• Protein Accession #: NP_001026097.1
• Nucleotide Accession #: NM_001030926.1