DDX3Y Antibody (OAGA00789)

Catalog No: OAGA00789

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
B: Hep G2
7.5% SDS PAGE
OAGA00789 diluted at 1:10000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA00789 diluted at 1:1000

DDX3 antibody detects DDX3 protein by Western blot analysis.
A. 30 ug Jurkat
B. 30 ug Raji
C. 30 ug NCI-H929
7.5 % SDS-PAGE
DDX3 antibody (OAGA00789) dilution: 1:10000

DDX3 antibody detects DDX3Y protein by Western blot analysis.
A. 50 ug Rat testis lysate/extract
7.5 % SDS-PAGE
DDX3 antibody (OAGA00789) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3Y(OAGA00789) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC/IF, WB
  Cellular Localization Cytoplasm, Nucleus
  Positive Controls H1299, HepG2, mouse brain, rat testis, Jurkat, Raji, NCI-H929
  
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 70 and 346 of DDX3 (Uniprot ID#O15523)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.77 mg/ml
• Intended Use: For in vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
• Application Info: WB 1:1000~10000
  ICC/IF 1:100~1000
  Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: DDX3Y
• Gene Full Name: ATP-dependent RNA helicase DDX3Y
• Alias Symbols: DBY
• NCBI Gene Id: 8653
• Protein Name: ATP-dependent RNA helicase DDX3Y
• Description of Target: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. The gene mutation causes male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that this gene plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
• Uniprot ID: O15523
• Protein Accession #: NP_001116137.1
• Molecular Weight: 73 kDa