DDX39B Antibody (OAGA01605)

Catalog No: OAGA01605

Size:100 ul

BAT1 antibody detects BAT1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BAT1 antibody (OAGA01605) diluted by 1:500.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UAP56 (OAGA01605) antibody at 1:100 dilution.

UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
UAP56 antibody [N1C2] (OAGA01605) diluted at 1:500.

UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
UAP56 antibody [N1C2] (OAGA01605) diluted at 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UAP56 (OAGA01605) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UAP56. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.43mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: DDX39B
• Gene Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
• Alias Symbols: BAT1, UAP56, D6S81E
• NCBI Gene Id: 7919
• Description of Target: This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene.
• Uniprot ID: Q13838