DDX21 Antibody - C-terminal region (ARP36375_T100)

Catalog No: ARP36375_T100

WB Suggested Anti-DDX21 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysateDDX21 is supported by BioGPS gene expression data to be expressed in HepG2

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX21
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Pig: 93%; Rabbit: 93%; Rat: 93%
• Peptide Sequence: Synthetic peptide located within the following region: FRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-DDX21 (ARP36375_T100) antibody is Catalog # AAP36375 (Previous Catalog # AAPP08433)
• Sample Type Confirmation:

  DDX21 is supported by BioGPS gene expression data to be expressed in HepG2

• Reference: Henning,D., et al., (2003) J. Biol. Chem. 278 (52), 52307-52314
• Publications:

  Miyazawa, N. et al. Human cell growth regulator Ly-1 antibody reactive homologue accelerates processing of preribosomal RNA. Genes Cells 19, 273-86 (2014). 24495227

  Romanova, L. et al. Critical role of nucleostemin in pre-rRNA processing. J. Biol. Chem. 284, 4968-77 (2009). 19106111

Target Info

• Gene Symbol: DDX21
• Gene Full Name: DEAD (Asp-Glu-Ala-Asp) box helicase 21
• Alias Symbols: GUA, GURDB, RH-II/GU, RH-II/GuA
• NCBI Gene Id: 9188
• Protein Name: Nucleolar RNA helicase 2
• Description of Target: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX21 encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
• Uniprot ID: Q9NR30
• Protein Accession #: NP_004719
• Nucleotide Accession #: NM_004728
• Protein Size (# AA): 783
• Molecular Weight: 86kDa
• Protein Interactions: HUWE1; PA2G4; KPNA3; CEP250; SUMO2; SUMO3; STAU1; IVNS1ABP; UBC; MDM2; LGR4; SUMO1; ERG; WWOX; EED; rev; SRPK2; SRPK3; FBXO6; TARDBP; LYN; AICDA; PAN2; vif; WHSC1; VCAM1; ITGA4; IL7R; FN1; CSNK2A1; YWHAE; SMURF1; H2AFX; APTX; ESR1; FTSJ3; PCDHA2; RRP7A; R