DDX18 Antibody (OAGA02138)

Catalog No: OAGA02138

Size:100 ul

DDX18 antibody detects DDX18 protein by Western blot analysis.
A. 30 ug Jurkat whole cell lysate/extract
B. 30 ug Raji whole cell lysate/extract
C. 30 ug NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
DDX18 antibody (OAGA02138) dilution: 1:1000

DDX18 antibody detects DDX18 protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: DDX18 protein stained by DDX18 antibody (OAGA02138) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DDX18. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
  

Target Info

• Gene Symbol: DDX18
• Gene Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
• Alias Symbols: Has1, MrDb
• NCBI Gene Id: 8886
• Description of Target: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein.
• Uniprot ID: Q9NVP1