DDX1 Antibody - middle region (OAGA02498)

Catalog No: OAGA02498

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
7.5% SDS PAGE
OAGA02498 diluted at 1:1000

DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
DDX1 antibody [N3C2], Internal (OAGA02498) dilution: 1:500.

DDX1 antibody [N3C2], Internal detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
DDX1 antibody [N3C2], Internal (OAGA02498) dilution: 1:500.

Immunofluorescence analysis of methanol-fixed HeLa, using DDX1 (OAGA02498) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DDX1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.76mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: DDX1
• Gene Full Name: DEAD (Asp-Glu-Ala-Asp) box helicase 1
• Alias Symbols: DBP-RB, UKVH5d
• NCBI Gene Id: 1653
• Description of Target: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin.
• Uniprot ID: Q92499