DDT Polyclonal Antibody (OABB00403)

Catalog No: OABB00403 (Formerly GWB-BBP326)

Size:100UG

Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Pancreas Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Lung Tissue Lysate

IHC(P): Human Placenta Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
  Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Reference: 1. Esumi, N., Budarf, M., Ciccarelli, L., Sellinger, B., Kozak, C. A., Wistow, G.Conserved gene
  structure and genomic linkage for D-dopachrome tautomerase (DDT) and MIF.Mammalian Genome
  9: 753-757, 1998.
  2. Nishihira, J., Fujinaga, M., Kuriyama, T., Suzuki, M., Sugimoto, H., Nakagawa, A., Tanaka, I., Sakai,
  M.Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for
  enzyme activation.Biochem. Biophys. Res. Commun. 243: 538-544, 1998.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: DDT
• Gene Full Name: D-dopachrome tautomerase
• Alias Symbols: DDCT;D-dopachrome decarboxylase;D-dopachrome tautomerase;D-DT;MIF2;MIF-2;phenylpyruvate tautomerase II.
• NCBI Gene Id: 1652
• Protein Name: D-dopachrome decarboxylase
• Description of Target: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
• Uniprot ID: P30046
• Molecular Weight: 12712 MW