Catalog No: OKEH00729
Size:96T
Price: $525.00
SKU
OKEH00729
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology DDT ELISA Kit (Human) (OKEH00729) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human DDT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DDT and incubated. The DDT found in the sample or standards competes with the biotinylated DDT for limited binding sites on the immobilized anti-human DDT antibody. Excess unbound biotinylated DDT and sample or standard DDT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DDT captured in the well and inversely proportional to the amount of DDT which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.9% (n = 20) Mean Inter-assay CV%: < 9.3% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.12 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human D-dopachrome decarboxylase | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | DDT |
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Gene Full Name | D-dopachrome tautomerase |
Alias Symbols | DDCT;D-dopachrome decarboxylase;D-dopachrome tautomerase;D-DT;MIF2;MIF-2;phenylpyruvate tautomerase II. |
NCBI Gene Id | 1652 |
Protein Name | P30046 |
Description of Target | D-dopachrome decarboxylase |
Uniprot ID | https://www.uniprot.org/uniprot/P30046 |
Protein Accession # | NP_001077861.1 |
Nucleotide Accession # | NM_001084392.1 |
Protein Size (# AA) | 118 |
Molecular Weight | 12 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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