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Catalog No: OKEH05437
Size:96T
Price: $525.00
SKU
OKEH05437
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ddr2 ELISA Kit (Mouse) (OKEH05437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ddr2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ddr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ddr2 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 94.6%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <8.3%, n = 20
Mean Inter-Assay Precision - <10.1%, n = 20
ELISA Kit Component
ComponentAmount
DDR2 Microplate96 Wells (12 x 8 Well strips)
DDR2 Lyophilized Standard2
100X Biotinylated DDR2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.093 ng/mL
SpecificityNatural and recombinant Mouse Discoidin domain-containing receptor 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDdr2
Gene Full Namediscoidin domain receptor family, member 2
Alias SymbolsAW495251;CD167 antigen-like family member B;discoidin domain receptor 2;discoidin domain-containing receptor 2;neurotrophic tyrosine kinase, receptor-related 3;Ntr;Ntrkr3;receptor protein-tyrosine kinase TKT;tyro10;tyrosine-protein kinase TYRO10.
NCBI Gene Id18214
Protein NameQ62371
Description of TargetDiscoidin domain-containing receptor 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q62371
Protein Accession #NP_072075.2
Nucleotide Accession #NM_022563.2
Protein Size (# AA)854
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