Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD00559 (Formerly GWB-KBBBU8)
Size:96WELLS
Price: $675.00
SKU
OKCD00559
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for DDO ELISA Kit (Human) (OKCD00559)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Aspartate Oxidase (DDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Aspartate Oxidase (DDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Aspartate Oxidase (DDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of D-Aspartate Oxidase (DDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Aspartate Oxidase (DDO) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Aspartate Oxidase (DDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DDO Microplate96 Wells (12 x 8 Well Strips)
DDO Lyophilized Standard2 x 10 ng
100X Biotinylated DDO Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of D-Aspartate Oxidase (DDO). No significant cross-reactivity or interference between D-Aspartate Oxidase (DDO) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolDDO
Gene Full NameD-aspartate oxidase
Alias Symbolsaspartic oxidase;DASOX;D-aspartate oxidase;D-aspartate oxidase, DDO;DDO-1;DDO-2.
NCBI Gene Id8528
Protein NameD-aspartate oxidase
Description of TargetSelectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.
Uniprot IDQ99489
Protein Accession #NP_003640
Nucleotide Accession #NM_003649.2
Protein Size (# AA)341
Write Your Own Review
You're reviewing:DDO ELISA Kit (Human) (OKCD00559)
Your Rating
We found other products you might like!