DDIT4 ELISA Kit (Human) (OKCA00657)

Catalog No: OKCA00657

Size:96 Wells

Human DDIT4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	89	86-92
  1:2	94	89-98
  1:4	96	92-99
  1:8	98	92-103

• ELISA Kit Principle: Aviva Systems Biology DDIT4 ELISA Kit (Human) (OKCA00657) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DDIT4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DDIT4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DDIT4 captured in the well.
• ELISA Kit Range: 0.156 ng/mL-10 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94	89-99

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level DDIT4 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level DDIT4 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • DDIT4 Microplate: 96 Wells (12 x 8 Well strips)
  • DDIT4 Lyophilized Standard: 2
  • 100X Biotinylated DDIT4 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Tissue Homogenates, Cell Lysates, Urine, Cerebrospinal Fluid (Csf)
• Sensitivity: 0.039 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: DDIT4
• Gene Full Name: DNA damage inducible transcript 4
• Alias Symbols: Dig2, REDD1, REDD-1
• NCBI Gene Id: 54541
• Protein Name: DNA damage-inducible transcript 4 protein
• Description of Target: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Required for mTORC1-mediated defense against viral protein synthesis and virus replication. Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
• Uniprot ID: Q9NX09
• Protein Accession #: NP_061931.1
• Nucleotide Accession #: NM_019058.2