DDIT4 Antibody - C-terminal region (OAAB16597)

Catalog No: OAAB16597

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Western blot analysis of DDIT4 (arrow) using rabbit polyclonal DDIT4 Antibody (C-term) (Cat.# OAAB16597). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDIT4 gene.

DDIT4 is strongly supported by BioGPS gene expression data to be expressed in HEK293

DDIT4 Antibody (C-term) (Cat. # OAAB16597) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDIT4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DDIT4 Antibody (C-term) (Cat. # OAAB16597) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity) Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
  Cellular Location: Mitochondrion (By similarity). Cytoplasm, cytosol
  Tissue Location: Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This DDIT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the C-terminal region of human DDIT4.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Cow; Mouse; Rat
• Concentration: 2 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: DDIT4
• Alias Symbols: Dig2, REDD1, REDD-1
• NCBI Gene Id: 54541
• Protein Name: DNA damage-inducible transcript 4 protein
• Uniprot ID: Q9NX09
• Protein Accession #: NP_061931.1
• Molecular Weight: 25371 Da