Catalog No: OPPA01740 (Formerly GWB-AB78E7)
Size:5UG
Price: $75.00
SKU
OPPA01740
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA01740 |
---|
Predicted Species Reactivity | Human |
---|---|
Product Format | The DDIT3 protein solution (1mg/ml) contains 20mM Tris-HCl pH-8 and 20% glycerol. Physical appearance: Sterile Filtered colorless solution. |
Host | E. Coli |
:: | Product Introduction: DDIT3 reduces DNA-binding activity of C/EBP and lap by forming heterodimers which don't bind DNA. DDIT3, also known as GADD153, is a basic domain-leucine zipper(bZIP) transcription factor of C/EBP family. DDIT3 protein is up-regulated by several stresses, such as amino acid or glucose starvation, endoplasmic reticulum (ER) stress, osmotic stress and hypoxia. DDIT3 protein is invloved in ER stress-mediated apoptosis and in disease including diabetes, brain ischemia and neurodegenerative disease. DDIT3 plays a role in asoprisnil-induced apoptosis. Hypoglycaemia-induced necrotic cell death of neuroblastoma cells is an active process mediated via the induction of the transcription factor DDIT3. DDIT3 plays an important role in melanoma progression. HRG stimulation of mammary epithelial cells induces the expression of DDIT3 mRNA and protein and transcription of DDIT3 promoter. Product Description: DDIT3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids and having a molecular mass of 21 kDa. The DDIT3 protein is fused to a 20 amino acids His tag at N-terminus.The DDIT3 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | DDIT3 although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | MGSSHHHHHH SSGLVPRGSH MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA. |
Gene Symbol | DDIT3 |
---|---|
Alias Symbols | CHOP, CEBPZ, CHOP10, CHOP-10, GADD153, AltDDIT3, C/EBPzeta |
NCBI Gene Id | 1649 |
Protein Name | DNA damage-inducible transcript 3 protein |
Description of Target | DDIT3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids and having a molecular mass of 21 kDa. The DDIT3 protein is fused to a 20 amino acids His tag at N-terminus. The DDIT3 is purified by proprietary chromatographic techniques. |
Uniprot ID | P35638 |
Protein Accession # | NP_004074.2 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review
We found other products you might like!