Catalog No: OKEH05497
Size:96T
Price: $625.00
SKU
OKEH05497
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ddc ELISA Kit (Mouse) (OKEH05497) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ddc has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ddc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ddc captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - < 6.4%, n = 20
Mean Inter-Assay Precision - < 9.0%, n = 20
ELISA Kit Component
ComponentAmount
DDC Microplate96 Wells (12 x 8 Well strips)
DDC Lyophilized Standard2
100X Biotinylated DDC Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.092 ng/mL
SpecificityNatural and recombinant Mouse Aromatic-L-amino-acid decarboxylase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolDdc
Gene Full Namedopa decarboxylase
Alias SymbolsAa;Aadc;aromatic-L-amino-acid decarboxylase;DOPA decarboxylase.
NCBI Gene Id13195
Protein NameO88533
Description of TargetAromatic-L-amino-acid decarboxylase
Uniprot IDhttps://www.uniprot.org/uniprot/O88533
Protein Accession #NP_001177377.1
Nucleotide Accession #NM_001190448.1
Protein Size (# AA)480
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